Figure 2

GM1 and Galgt1 expression in dystrophic skeletal muscle. (A) Immunostaining of GM1 ganglioside and Galgt1 protein in 6-week-old mdx and Galgt1 ^−/− mdx skeletal muscle. (B) qRT-PCR comparison of Galgt1 gene expression in WT and mdx skeletal muscle at 6 weeks and 3 months of age. (C) GM1 and secondary antibody (control) staining in muscle sections obtained from patients with Duchenne muscular dystrophy (DMD) or from otherwise normal human muscle. Errors in (B) are SEM averaged from six muscles per condition with two to three measures per muscle. ***P < 0.001, Bar is 50 μm.