Figure 2

CSQ1 Ca ²⁺ binding capacity. The ⁴⁵Ca²⁺ binding in a spin dialysis binding assay was carried out using 200 μg protein (at a concentration of 1 mg/ml) in 150 mM NaCl, 20 mM MOPS, 100 μM to 5 mM CaCl₂, and 2.6 kBq ⁴⁵Ca²⁺. Each data point is the mean ⁴⁵Ca²⁺ bound, in nmol Ca²⁺/mg CSQ, and the bars are SEM for each of the CSQ1 constructs, under the Ca²⁺ conditions listed. Asterisks (*) indicate a significant difference in ⁴⁵Ca²⁺ bound to CSQ1 constructs compared to WT CSQ1 (P < 0.05). N = 5 to 9.