Figure 3

Lack of PGC-1α results in reduced lysososmal abundance and denervation-induced autophagy flux. (A-C) Blots and quantification of autophagic proteins in control (Con), denervated (Den) WT, and PGC-1α KO (KO) animals; treated with vehicle (water) or 0.4mg/kg/day colchicine (col) for 4 days. (A) Representative blots. Quantification of (B) LC3BII and (C) p62, (D) Representative blot and quantification of Nix, (E) basal autophagy flux, and (F) denervation-induced autophagy flux, (G) Confocal images of fixed single fibers immuno-stained for LC3 (green) and lysosomal Lamp-2 (red) and colocalization is shown in yellow (Merge) and represents autophagosomes within lysosomes. Nuclei are in blue (DAPI). *P < 0.05 significant difference between Con and Den. †P < 0.05 significant effect of genotype. GAPDH was used as a loading control (n = 3 to 5 for all groups). AU, arbitrary units; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; KO, knockout; WT, wild type.