Fig. 7
From: Akt-mediated phosphorylation controls the activity of the Y-box protein MSY3 in skeletal muscle
Disruption of Akt substrate Ser126 alters MSY3 nuclear/cytoplasmatic trafficking and function in myogenic cells. a Top: FLAG expression in C2C12 myoblasts transiently transfected with FLAG-tagged MSY3 proteins mutated at Ser126 (FLAGSer126Ala) and at Ser 328 (FLAGSer328Ala). Scale bar = 150 μm. Bottom: Western blot with anti-FLAG Ab of protein extracts of C2C12 transiently transfected with FLAG-tagged MSY3 protein (FLAGMSY3), FLAGSer126Ala and FLAGSer328Ala. b Measurements of myogenin expression by qRT-PCR in myrsAkt transfected C2C12 multiclones expressing FLAG MSY3, FLAGΔCSD, and FLAGSer126Ala transfected with myristoylated Akt (Akt) in proliferation medium (GM) or upon different times cultured in differentiation medium (24–72 h DM). GAPDH was used to normalize expression levels