Fig. 7
From: A differential response to newt regeneration extract by C2C12 and primary mammalian muscle cells
Injection of newt extract directly into C2C12 myotubes. Myotubes were microinjected with either DMEM/GFP (control) or Extract/GFP. a Arrows identify GFP-positive cells that were also positive for Ki67 (a proliferation marker). b Cell counts showed that greater than 40 % of the injected myotubes were positive for Ki67. There were also GFP/Ki67-positive myocytes. Since only multinucleated cells were microinjected, the presence of GFP-positive myocytes suggests that fragmentation may have occurred. c RT-PCR of microinjected cultures showed a significant downregulation of myogenin in extract-treated cultures. *p < 0.05, **p < 0.0001