Fig. 5

Cytokines secreted in myoblast-macrophage co-cultures. ELISA analysis of 3-day old WT co-culture supernatants showing expression of IL-10 (a) and IL-12 (b) in differentially polarized macrophages from WT (black bars) or Bla/J (white bars) mice. c Cytokine arrays performed on supernatants of 3-day-old WT myoblasts co-cultured without macrophages (white) or with WT mice-derived Mϕ (black), M1 (light grey), or M2a (dark grey) macrophages showing expression of M1-specific cytokines. d MSD IL-4 assay performed on supernatants of 1-day-old WT (white) or A/J (black) myoblasts that were either cultured alone (none) or co-cultured with WT mice-derived M1 or M2a macrophages. Data are shown as means ± SEM. n = 3; *P < 0.05; **P < 0.01; ***P < 0.001