Fig. 5
dy2J Mice treated with MABs + mMAG show improved locomotion. a Schematic representation of cell treatment, functional, and histological analyses. b Treadmill analysis in ten dy2J mice untreated (saline solution, blue, group 1), ten treated with MABs + mMAG (green, group 2), and ten treated with MABs alone (red, group 3) Average times (±SEM) measured on the treadmill before and after treatment are shown on the left. On the right, the percentage of deterioration between the two measurements is depicted. dy2J Mice treated with MABs + mMAG deteriorate significantly less than untreated dy2J mice (**p < 0.01; n = 10; ANOVA). dy2J Mice treated with MABs alone behave similar to controls. c Fibrosis revealed by Sirius red staining of the tibialis anterior muscle in dy2J mice untreated (saline solution, blue), treated with MABs + mMAG (green), and treated with MABs alone (red); on the right, representative staining of the three groups as compared to normal wild type mice. The area of fibrosis is significantly reduced in dy2J mice treated with MABs + mMAG as compared to dy2J mice treated with saline or MABs alone. d Morphometric analysis of the tibialis anterior muscle from dy2J mice untreated (saline, blue), treated with MABs + mMAG (green), or with MABs alone (red). The histogram shows that the fiber size distribution of dy2J mice treated with MABs + mMAG is shifted to the right as compared to mice untreated or treated with MABs alone, indicating a higher percentage of fibers with larger diameter. Below, the representative H&E staining of muscle histology from the tibialis anterior of the three groups of dy2J mice. Muscle atrophy and endomysial connective tissue is prevalent in untreated muscle and in muscle treated with MABs alone. Reduced muscle fiber size variability and connective tissue is evident in muscle treated with MABs + mMAG. Scale bar = 50 μm