Fig. 3

Contractile properties of transplanted muscles and satellite cell homing. a Representative examples of maximum isometric tetanic force in TA muscles that had been injected with PBS (contra-lateral leg, gray line) and control or Pax3-induced cells (red lines). Wild-type Bl6 mice were used for reference control (dashed line). b, c Cell transplantation produces an improvement in absolute (F ₀, b) and specific (sF ₀ = F ₀ normalized to CSA, c) force. Error bars represent s.e.m. from a total of six mice. *P < 0.05, **P < 0.01, ***P < 0.001. d In situ analysis reveals the presence of donor-derived satellite cells (ZsGreen/Pax3-induced cells) in the host stem cell pool, as shown by the presence of cells co-stained for both Pax7 (red) and ZsGreen (green) (white arrow) beneath the basal lamina (gray). e Upon reinjury, engrafted donor-derived satellite cells give rise to newly formed myofibers, as indicated by the co-expression of DYS (red) and embryonic MHC (green) (white arrow). Arrowheads denote DYS⁻/eMHC⁺ host-derived new formed myofibers. DAPI is shown in blue. Scale bar, 50 μm