Fig. 4

Characterization of the muscular phenotype in the Ano5 KO mice. a Tibialis anterior (TA) and quadriceps (Qu) muscle mass (MM) normalized to body weight (BW) from the indicated genotypes of mice. b H&E-stained cryosections of the gastrocnemius (GA) and quadriceps (Qu) from 18-month-old mice of the indicated genotypes. Scale bar = 50 μm. c Representative immunofluorescence images for caveolin-3 (green) and nuclei (DAPI, blue) staining in gastrocnemius and quadriceps sections from 18-month-old WT and KO mice. Scale bar = 100 μm. d Percentage of myofibers with centrally located nuclei from the gastrocnemius and quadriceps muscles of WT and KO mice at 18 months of age. ns, no statistical significance. N of mice: 6–8 for each group