Fig. 7

Validation of p38α binding at several identified targets. a Proliferating (GM) or differentiating (24 and 48 h DM) C2C12 cells were subjected to ChIP analysis with antibodies to p38α or control rabbit IgG. Immunopurified DNA was subjected to semiquantitative PCR with indicated primers, and the PCR products were then run on an agarose gel. Representative images are shown. Densitometry of the bands was measured using ImageJ and normalized to input. b, c DNA from C2C12 cells was immunopurified as in (a) and then subjected to qPCR analysis with indicated primers. Data show the relative recruitment of p38α at these promoters and represent the mean of three independent experiments ± SEM. Non-parametric Mann–Whitney U test was used to assess statistical significance (P value <0.05). d, e Proliferating (GM) or differentiating (24 and 48 h DM) C2C12 cells were subjected to ChIP analysis with antibodies to MyoD, Mef2 or control rabbit IgG and immunopurified DNA was subjected to qPCR analysis with indicated primers