Fig. 8
From: Characterization of a Dmd EGFP reporter mouse as a tool to investigate dystrophin expression
Dystrophin-EGFP expression of satellite cell-derived myotubes. Dystrophin-EGFP expression of in vitro differentiated satellite cells; isolated single fibers with their attached satellite cells from wild-type (WT) and Dmd EGFP mice were cultured for 4Â days and differentiated for a further 8Â days. Forming myotubes were fixed and stained with anti-GFP (green) and Dys2 (red) antibodies and counterstained with DAPI (blue)