Fig. 1

Pharmacological blockade of p38α signaling precludes unleashing of MyoD-associated HMT activity during myoblasts differentiation. a MyoD or control IgG immunoprecipitates retrieved from cell extracts of C2C12 grown in GM or DM with or without SB or LY were subjected to HMT activity assay. Methylated and input H3(N) were detected by fluorography and Commassie, respectively. b Luciferase activity was assessed in C2-4RE-luc reporter cells grown in GM or DM without or with SB or LY. Luciferase activity was expressed after protein normalization as fold activation. Error bar, ±SEM (n = 3). c Western blot analysis of primary HsMB cells expressing shRNA of scramble control shRNA (Ctrl) or p38α via lentiviral delivery grown in GM or DM with or without SB, probed with antibodies for indicated proteins. β-actin served as loading control