Fig. 5
From: Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle
Tamoxifen-induced conditional muscle specific Six1 knockout reduced fast-type gene expression in soleus. a Six1 mRNA expression levels in SOL of 3 month-old control (Ctrl, n = 3) and ciSix1 KO (n = 4) mice 1 month after tamoxifen injection. b Percentage of myofibers expressing MyHCI or MyHCIIA in SOL of 3 month-old control (Ctrl, n = 3) and ciSix1 KO (n = 4) mice 1 month after tamoxifen injection. c Immunostaining of MyHCI (red) and MyHCIIA (red) in SOL of 3 month-old control and ciSix1 KO mice 1 month after tamoxifen injection. Arrows indicate fibers expressing MyHCIIA, and an arrow head indicates a fiber expressing both MyHCI and MyHCIIA in ciSix1 KO mice. d-g mRNA expression levels of sarcomeric genes (d), energy metabolism (e), calcium regulation (f), transcription factors (g), and most down-regulated or up-regulated genes (h) in SOL of 3 month-old control (Ctrl, n = 3) and ciSix1 KO (n = 4) mice 1 month after tamoxifen injection. *P < 0.05, **P < 0.01, ***P < 0.001