Fig. 4
From: Direct reprogramming of urine-derived cells with inducible MyoD for modeling human muscle disease
Maturation of myotubes at 28-day and 35-day time points. a Bright-field images of myotube formation at the 28-day time point in iMyoD lines 2 and 11 after induction and differentiation (left panels). Middle panels show images of dystrophin (green) and α-actinin (red) protein expression, taken in the same field as the bright-field panels. The right panels show the merged images revealing dystrophin and α-actinin expressions throughout the lengths of the myotubes. Scale bar = 100 μm. b Images of iMyoD-induced UDCs after 35 days of differentiation showing expression of fast MHC, α-actinin, and titin. The magnified region of interest (ROI) is outlined in the first panels (white rectangles). White arrows highlight the membrane localization of dystrophin (green). White arrowheads highlight the formation of sarcomeres (red). Scale bar = 25 μm