Fig. 1
VAChT transcripts are increased in the spinal cord of VAChThyp compared to control mice (a). VAChT protein is also increased in motor axon nerve endings innervating skeletal muscles in VAChTHyp compared to control mice (b–d). Only male mice on a C57BL/6 background were used in these experiments. For qPCR, animals were 83 days old (n = 4 for control; n = 4 VAChThyp). For NMJ analysis, animals were 9 days old (n = 4 for control; n = 4 VAChThyp). Scale bar = 10 μm. Error bar = Standard Error. **P value <0.01