Fig. 6

Inhaled low-dose carbon monoxide protects against dystrophy. a Schematic of HO-1 enzymatic action. b Mice were placed in chambers containing 250 ppm CO for 1 h per day. c, d Serum creatine kinase (c) and sample Evans blue staining in diaphragm and % Evans blue extravasation in gastrocnemius (d) in mdx mice exposed to CO versus air alone. e Relative expression of the indicated genes in gastrocnemius from mdx mice exposed to CO versus air alone. f, g Fiber size distribution in gastrocnemius (f) and % central nuclei in gastrocnemius muscle (g) from mdx mice exposed to CO versus air alone. n = 8. *P < 0.05, Student’s t test. Scale bar = 50 μm. Error bars indicate ±SEM