Fig. 4

Expansion of Dba satellite cell-derived myoblasts on laminin 521 maintains differentiation when transferred to alternate substrates. Passage 8 myoblasts were transferred from laminin 521 to alternate substrates (a) but still show robust differentiation visualized by MHC expression (green) and Hoechst (blue). MHC area was quantified for all the different expansion and differentiation conditions (b). Laminin 521 expanded cells differentiated on all substrates. Error bars represent ±S.D. Scale bar represents 200 μM. Statistical significance was determined by one-way ANOVA with Bonferroni correction. N = 10 technical replicates from one biological sample. Significance is annotated as less than .01 (**), less than .001 (***), and less than .0001 (****)