Fig. 4

Estimated adipocyte volumes are similar between intact and decelluarized 5th toe EDL muscles. a Representative sequential histology demonstrating lipid withdrawal (arrows) and fractionation (asterisks) artifacts incurred by sectioning and ORO staining. Perilipin immunofluorescent signal (red, center) marks where lipid membranes remain fixed and eosin staining (pink, right) shows fiber material where ORO staining marked lipid. b Histogram of adipocyte volumes estimated from sequential perilipin and H&E staining of serial sections of intact muscle (combined data from four sampled muscle volumes). c Histogram of adipocyte volumes estimated from fluorescent confocal imaging of decellularized muscles (combined data from four muscles)