Fig. 5

Altered paxillin and FAK distribution in SLK-deficient muscles. a, d Vinculin immunostaining remained unchanged between wild type and knockout fibers in TA muscles. However, paxillin (b, e) was readily detectable at the periphery of wild-type myofibers but displayed a cytosolic pattern in knockouts. c, f FAK localization was also severely disrupted in knockout fibers with no cytoplasmic staining and strong positive clusters underneath the membrane. g Western blot analysis for markers of the focal adhesion complex showed no differences in the activation status of either FAK or Paxillin. h Illustration of myofibers showing viewpoints of confocal images. i, j Confocal image of paxillin staining in wild type and knockout fibers