Fig. 3
Changes in embryonic myosin heavy chain expression (eMyHC) and muscle fiber number per whole cross section in young versus mature satellite cell-replete (SC+) and -depleted (SC-) mice after synergist ablation overload of the plantaris for 10 days (OV). a eMyHC expression after OV in young (n = 8 SC+, n = 6 SC-) and mature (n = 7 SC+, n = 7 SC-) mice, *p < 0.05 SC+ versus SC- OV within each age group. b IHC representative image visualizing eMyHC fibers of differing size in a mature SC+ mouse; green arrow points to a small-caliber eMyHC+ muscle fiber <300 μm2. c Muscle fiber number in young sham (n = 7 SC+, n = 6 SC-) and OV (n = 8 SC+, n = 6 SC-) mice. d Muscle fiber number in mature sham (n = 9 SC+, n = 7 SC-) and OV (n = 6 SC+, n = 7 SC-) mice. *p < 0.05 compared to respective sham. e–f Representative image showing central nuclei (white arrows) in eMyHC+ (green) and eMyHC- (unstained) muscle fibers (pink borders). Scale bar=50 μm