Fig. 2

Impaired activation of caspases in skeletal muscle of MKP-5-deficient mice in response to injury. (a, b) Muscle lysates prepared from mkp-5 ^+/+ and mkp-5 ^−/− mice were immunoblotted for the expression of the cleaved (active) forms of caspase-3 in soleus muscle at 12 h after CTX-induced injury. The expression of the cleaved (active) forms of caspase-3, caspase-8 and caspase-9 was measured in soleus muscle at 10 days after CTX-induced injury (c, d, e, f). Graphs represent quantitation of the cleaved form of the indicated caspases normalized to Erk1/2. Values are means ± standard errors of the means (SEM). **P < 0.01, n = 5 per genotype