Fig. 4
From: The breaking and making of healthy adult human skeletal muscle in vivo
Detailed three-dimensional view of single fibres. Representative confocal microscopy images of single fibres from regenerating human skeletal muscle 7 days post injury. An intact uninjured fibre lies between 2 injured fibres. Eight individual slices (numbered) of a stack are displayed, with the 4 channels separated into basement membrane (collagen IV, yellow) and nuclei (blue), or macrophages (CD68, green) and desmin (red). Slice 5 is presented with 4 colours merged and single channel grey scale images below. The thinner regenerating zones (arrows) of the injured fibres contain macrophages and desmin+ cells and filaments, within the basement membrane. Both cell types are also present in the wider necrotic zones (asterisk) but only at the periphery (seen in slices 2 and 7), surrounding an acellular core (slices 4–5). Note macrophages in particular can be seen entirely cuffing the necrotic zones in the x, y, and z planes. Scale bars, 200 μm