Fig. 5

Proteomic studies to unravel the molecular nature of p.P104L induced muscular dystrophy in mice. a Proteomic workflow applied in this study. b Results of our label-free shotgun proteomic profiling are shown as a volcano plot. All points (each one represents a protein) over the horizontal line have a statistically significant p-ANOVA of ≤ 0.05. In total, 43 proteins are decreased (red points) and 77 proteins are increased in abundance (green points) in mutant mouse muscle. c Results of an in silico pathway analysis of proteins altered in abundance confirm alterations of the protein processing machinery, vulnerability of mitochondria as well as altered in ECM protein processing and cellular metabolism. In addition, there is evidence of oxidative stress and sarcolemmal vulnerability