Fig. 6
From: Deletion of the microtubule-associated protein 6 (MAP6) results in skeletal muscle dysfunction
Calcium release is reduced in MAP6 KO myotubes compared to WT. a Direct stimulation of RyR1 by 500 μM 4CmC (black arrowhead). Fluorescence variations as a function of time are represented for WT myotubes (white dots) and MAP6 KO myotubes (black dots). Values are represented as means ± SEM for n = 150 and 151 myotubes respectively, from three different experiments, Student’s t test, ns: non-significant. b Membrane depolarization induced by addition of 140 mM KCl (black arrowhead). Fluorescence variations are presented for WT and MAP6 KO myotubes. Values are means ± SEM for n = 120 and 110 myotubes respectively, from three different experiments. Student’s t test, ***p < 0.001. c, d Maximal amplitude and area under curve for 4CmC stimulation (c) and KCl stimulation (d), with the number of analyzed-myotubes in each bar of the plot. Values are represented as means ± SEM. Student’s t test, ****p < 0.0001, **p < 0.01; ns: non-significant