Fig. 4

Examples of correctly and incorrectly processed muscle images. a Representative pseudo-colored images from all five WT and mdx mice after processed by CellProfiler. Red, centrally nucleated fibers; blue, normal fiber. b An incorrectly processed image with a large black area, which was due to a high threshold setting and can be corrected by lowering the threshold from 0.985 to 0.955. The blue stars showing the inter-fiber spaces that were mistakenly identified as muscle fibers; the blue boxes indicating individual muscle fibers that were mistakenly split into two