Fig. 1

ZFP36L1 and ZFP36L2 are both required for whole body growth. Characterisation of Zfp36L1/L2-P mice. Controls represent Cre-negative littermates. a Western blot showing the ablation of ZFP36L1 and ZFP36L2 in isolated satellite cells from Zfp36L1/L2 mice (see also Additional file 1). b Weights of male and female Zfp36L1/L2-P and control mice measured from 10 days to 45 days of age. Error bars represent SEM, p < 0.0001**** measured by two-way ANOVA with Tukey’s multiple comparison test, n = 10. c Weights of tibialis anterior (TA) and gastrocnemius hind limb skeletal muscle from 3- to 4-week-old Zfp36L1/L2-P and control mice in milligrammes. Significance was measured by unpaired, two-tailed Mann Whitney test, n = 8 for control; n = 7 for Zfp36L1/L2-P. d Transverse cross sections of TA muscles were stained with haematoxylin (H; myofibre; pink) and eosin (E; nuclei; purple), and immunostained with antibodies against laminin (green), an extracellular matrix protein to delineate the periphery of the individual muscle fibres and with DAPI (blue), a nuclear marker. Scale bars: 100 μm. H and E images were acquired using a × 20 objective lens, and immunofluorescence images were acquired using a × 40 objective lens. Images representative of n = 3