Fig. 5

ASM deficiency impairs the rapid intracellular accumulation of caveolar vesicles after injury, resulting in abnormal fiber morphology. FDB fibers isolated from ASM-deficient mice (ASM^−/−) and their wild type littermates (WT) were left intact or injured by one passage through a 30-gauge needle. After 1 min at 37 °C, the fibers were fixed and processed for TEM. a Lower magnification TEM images of the overall ultrastructural morphology of WT or ASM^−/− fibers with or without wounding. Bars, 1 μm. b TEM images of three independent fibers per condition at higher magnification. The arrowheads point to caveolae on the sarcolemma, and the arrows point to intracellular caveolar vesicles. Bars, 200 nm. The images in a and b are representative of multiple images acquired from a total of approximately 40 individual fibers