Fig. 2
From: Metabolic derangements of skeletal muscle from a murine model of glioma cachexia
Atrophy and expression of E3 ubiquitin ligases in the gastrocnemius muscle in glioma-bearing mice at the time point after implantation. a Macroscopic observation of gastrocnemius muscles in cachectic mice (CHG5 and U87) relative to tumor-free mice (HEB). b Grip strengths of forelimbs monitored every 7 days. c Representative micrographs of HE histology of gastrocnemius muscles. Scale bars, 20 μm. d Quantification of the myofiber cross-sectional areas in glioma-bearing mice relative to HEB mice. e–g Expressions of two E3 ubiquitin ligases MuRF1 (f) and Atrogin1 (g) measured by western blot. Values that were significantly different from the control group were evaluated using one-way ANOVA. Data are presented as mean ± SD (*P < 0 .05; **P < 0.01; ***P < 0.001)