Fig. 3

The therapeutic effect of IMB0901 in the in vitro model. a The effect of IMB0901 on the expression of three myotube protein markers MyHC, MyoD, and MyoG in the in vitro cell model. Western blotting was used to detect the effect of IMB0901 on the expression of three myotube protein markers MyHC, MyoD, and MyoG during the process of C2C12 myotube atrophy induced by C26 cell supernatant. b The effect of IMB0901 on the cytomorphology of C2C12 myotube atrophy induced by C26 cell supernatant. Left: The cell morphology during the process of C2C12 myotube atrophy induced by C26 cell supernatant was recorded with the inverted microscopy (upper), and the MyHC expression in C2C12 myotubes was detected by immunofluorescence and the nuclei was stained by DAPI (down). Right: The number of nuclei per field was examined by Image J software (upper), and the myotube area percentage was calculated by the immunofluorescence area divided by the horizon area (down). The values presented means ± SD from three experiments. *P < 0.05 and **P < 0.01 compared to the control group, ^#P < 0.05 and ^##P < 0.01 compared to the CM group