Fig. 2

Matriglycan in the mouse RMS-derived cell line U23674. a–c Western blotting of WGA-enriched protein samples from the U23674 or C2C12 cell line, and from skeletal muscle (SkM), for core α-dystroglycan (a), matriglycan-modified α-dystroglycan (b), and laminin-bound dystroglycan (c). d Relative expression of mRNAs encoding dystroglycan (DAG1) and the dystroglycan-modifying glycosyltransferases in U23674 vs. C2C12 cells, as quantified by real-time PCR. The 12 genes tested are those known to be directly or indirectly involved in its functional glycosylation. Rpl27 was used as the normalizing control. U23674 gene expression was normalized to C2C12. * These values are statistically different according to unpaired two-sample t test, p < 0.05. e Matriglycan levels in U23674 cells infected with adeno-viral vectors expressing the dystroglycan-modifying glycosyltransferases, as assessed by OnCell Western assay using an anti-matriglycan antibody. The U48484 cell line serves as a positive control