Fig. 1

cIAP1 expression is induced in denervation-induced muscle atrophy. A small (5–10 mm) piece of the right sciatic nerve was removed to induce denervation of the entire right hind limb of 6-week-old female C57BL/6 mice. The contralateral leg served as an internal control. Mice were sacrificed 14 days post denervation. a Muscle mass of tibialis anterior (TA), soleus (Sol) and extensor digitorum longus (EDL) from non-denervated (black bars) or denervated (white bars) hind limbs. b Average fiber size of muscle fibers from non-denervated or denervated TA, Sol, and EDL muscles. c Representative Western blots of Atrogin-1, MuRF1, MyHC, cIAP1, and GAPDH protein expression in Gas muscle from non-denervated (N) and denervated (D) hind limbs. Quantification of d Atrogin-1, e MuRF1, f MyHC, and g cIAP1 protein expression normalized with total protein and relative to non-denervated (N) muscle. Data is the mean ± SEM, n = 4, *p < 0.05, ***p < 0.001