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Fig. 6 | Skeletal Muscle

Fig. 6

From: Muscle injury-induced hypoxia alters the proliferation and differentiation potentials of muscle resident stromal cells

Fig. 6

Hypoxia induces Smad1/5/8 phosphorylation and BMP9 expression in mrSCs. a Western blot analysis of phospho-Smad 1/5/8 and total Smad 1/5/8 in mrSCs cultured hypoxia or normoxic incubator conditions (called “Normoxia”). Hypoxia caused a marked and sustained activation of the Smad1/5/8 pathway even after 120 h. b Graph showing the expression of several osteogenic BMPs after a 24 h exposure to hypoxia. There was a significant increase (3.6-fold) in BMP9 expression by mrSCs cultured in hypoxia while the levels of BMP2 and BMP7 were not affected (mean ± SEM of four independent experiments; n = 4–6/experiment; ***p < 0.001). c Western blot analysis of BMP9 in mrSCs cultured in hypoxia or in normoxic incubator conditions. After 24 h of hypoxia, BMP9 was detected in mrSCs and was still expressed after 120 h

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Skeletal Muscle

ISSN: 2044-5040

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