Fig. 2

Srsf9 mRNA levels are inversely proportional to miR-1/206 levels. a Srsf9 mRNA levels decrease in differentiating C2C12 cells. Expression was assessed by qPCR in growing myoblasts (day 0) and in day 6 myotubes. Gapdh was the reference gene. Expression levels in the differentiating samples were normalized to myoblast levels. Myogenin (MyoG) was the positive control as its expression increases during differentiation. *p ≤ 0.05; **p ≤ 0.01 vs. D0 N = 3 independent cultures for all genes and time points. b miR-206 and miR-1 levels increase during mouse hindlimb skeletal muscle development. Expression was assessed by qPCR and normalized to sno202 in whole hindlimb for developmental time points and in the soleus from 6-month-old male mice. Fold change relative to 15.5 dpc is presented. N for 15.5 dpc, 17.5 dpc, 19.5 dpc, and adult was 4, 6, 6, and 4, respectively. ***p ≤ 0.001 vs. 15.5 dpc. c Srsf9 and Ccnd1 mRNAs decrease during mouse hindlimb skeletal muscle development. Expression was assessed by qPCR with 18S rRNA as the reference gene. Expression levels were normalized to 15.5 dpc. *p ≤ 0.05; **p ≤ 0.01 vs 15.5 dpc Ns were the same as in panel (b)