Fig. 3

NR supplementation, NAD⁺ salvage and mitochondrial function in H6PDKO muscle. a-d NAD⁺, NAD(H), total NAD(H) quantification and ratio of NAD⁺/NADH in TA of WT and H6PDKO +/− intraperitoneal (i.p.) Nicotinamide Riboside (NR) (n = 6-9). e qRT-PCR data of genes involved in the biosynthesis of NAD⁺ and salvage of NAM in WT and H6PDKO muscle +/− i.p. NR (n = 6-9). f qRT-PCR data of genes critical carnitine and fatty acids metabolism in WT and H6PDKO muscle +/− i.p. NR (n = 6-9). g-h Western blots and quantification of the NAM salvage protein NAMPT and the skeletal muscle specific protein NRK2 (n = 6). i High-resolution respirometry of fatty acid oxidation in permeabilised TA tissue from WT and H6PDKO i.p NR (n = 3). j High-resolution respiration for fatty acid oxidation using WT and H6PDKO after i.p NR permeabilised SOL (n = 3). k Western blots showing total lysine acetylation and IDH2 acetylation in WT and H6PDKO muscle +/− i.p NR. l Western blots showing ER stress regulator CHOP and protein folding factors PDI and ERO1a in WT and H6PDKO +/− i.p NR. (n = 6-9). *P < 0.05, **P < 0.01 and ***P < 0.001