Fig. 4

Molecular and phenotypic analysis of H6PD/NRK2 Double knockout mice. a-d NAD⁺, NADH, total NAD(H) and ratio of NAD⁺/NADH in WT, H6PDKO, NRK2KO and DKO (H6PDKO-NRK2KO) TA muscle (n = 3-6). e-g Skeletal muscle tissue weights from WT, H6PDKO, NRK2KO and H6-NRK2 Double Knockout (DKO) (n = 3-6). h qRT-PCR of NAD⁺ biosynthetic gene expression in WT, H6PDKO, NRK2 KO and DKO in TA (n = 3-6). i qRT-PCR of mitochondrial and acyl-CoA genes in TA of WT, H6PDKO, NRK2 KO and DKO (n = 3-6). j Western blots of total lysine acetylation, IDH2 and H3K56 acetylation in WT, H6PDKO, NRK2KO and DKO muscle protein lysates (n = 3-6). k Western blots of CHOP and protein folding factors PDI and ERO1a in WT, H6PDKO, NRK2 KO and DKO muscle lysates (n = 3-6). *P < 0.05, **P < 0.01 and ***P < 0.001