Fig. 7

Specific force in AAV-treated and vehicle-treated EDLs of CTRL and Neb cKO mice. a, b Force-frequency curves for Z-disk AAV mice at the 1× (a) and 3× (b) dosage. Left: There was a significant decrease in the force-frequency curve fit for the CTRL mice at both doses and a slight, but significant, increase for the Neb cKO mice at the 1× dose. That increase in the Neb cKO curve fit was lost in the 3× dose. Right: Quantitation of maximal (200 Hz) tetanic force. Paired t tests at both doses show a significant decrease in CTRL mice, but no significant increase in Neb cKO mice. (Paired t tests were used to compare maximal tetanic force.) c Percent change between AAV-treated and vehicle-treated EDL in the 1× (left) and 3× (right) dosage groups. There is a significant difference between the response of CTRL mice and the response of Neb cKO mice to the treatment in both dosage groups. Analyzing the change in a one-sample t test with a reference value of 0% change shows that AAV treatment does not affect force production in Neb cKO mice (p = 0.11 (1× dose) and 0.44 (3× dose)). (1×: n = 9, 11 mice; 3×: n = 4, 9 mice)