The ERG1a potassium channel increases basal intracellular calcium concentration and calpain activity in skeletal muscle cells

Table 1 Sequences of primers used for quantitative PCR

Primer name (mouse)	Primer sequence 5′–3′	Size (bp)^a	Tm (°C)	GC (%)	Amplicon size (bp)^a
Merg1a forward	cctcgacaccatcatccgca	20	59.6	55.0	145
Merg1a reverse	aggaaatcgcaggtgcaggg	20	60.3	60.0
18S subunit forward	cgccgctagaggtgaaattct	21	57.2	52.4	101
18S subunit reverse	agaacgaaagtcggaggttc	20	57.0	52.4
Calpain 1 forward	gctaccgtttgtctagcgtc	20	58.73	55.0	98
Calpain 1 reverse	taactcctctgtcatcctctggt	23	59.99	47.83
Calpain 2 forward	ttttgtgcggtgtttggtcc	20	59.83	50.0	107
Calpain 2 reverse	aactcagccacgaagcaagg	20	60.89	55.0
Calpain 3 forward	ttcacaggaggggtgacaga	20	60.11	55.0	122
Calpain 3 reverse	ttcgtgccatcgtcaatggag	21	61.01	52.38
Calpastatin forward	gccttggatgacctgataga	20	53.8	50.0	115
Calpastatin reverse	gtgcctcaaggtaggtagaa	20	53.7	50.0

ISSN: 2044-5040