Fig. 4

The moderate and severe FSHD-like mouse models show significant decline in treadmill performance. Mild, moderate, and severe FSHD-like mice were assessed for treadmill performance, as described in the methods. a The moderately affected female mice (red line) were assayed prior to TMX injection (D0) and 3, 6, 10, 16, 23, and 29 days post-injection (DPI) and compared with age-matched female bi-transgenic mice (green line) and female ACTA1-MCM mice similarly injected with TMX (blue line). b The moderately affected male mice (red line) were assayed prior to TMX injection (D0) and at 2, 6, 10, 14, 17, 21, 24, and 28 DPI and compared with aged-matched male bi-transgenic mice (green line) and male ACTA1-MCM mice similarly injected with TMX (blue line). c Severely affected female mice (red line) were assayed prior to TMX injection (D0) and 2, 5, and 7 DPI (# D8, mice were too affected to be assayed) and compared with female bi-transgenic mice (green line) and ACTA1-MCM mice similarly injected with TMX (blue line). d Severely affected male mice (red line) were assayed prior to TMX injection (D0) and 2, 5, 7, and 8 DPI and compared with age-matched male bi-transgenic mice (green line) and ACTA1-MCM mice similarly injected with TMX (blue line). Data are mean ± S.D. with significance, *p < 0.05, **p < 0.01, ***p < .001, calculated between bi-transgenic +TMX and bi-transgenic no TMX (green) or ACTA1-MCM +TMX (blue)