Fig. 4

Exposure to the adult endogenous environment does not influence the oxidative phenotype of PSC-derived myofibers. a Representative images show staining for MyHC isoforms (red) and dystrophin (green) in TA muscles from NSGmdx^4cv mice that had been injected with iPax3 or iPax7-derived re-isolated mononuclear cells (MNCs). PBS served as control. Nuclei were counterstained with DAPI (blue). Magnification bar, 100 μm. b Bar graphs show respective quantification (panel a), as indicated by the percentage of DYS+MyHC+ double positive with respect to total DYS+ myofibers for iPax3-injected MNCs (gray bar, secondary recipients) in comparison to data from iPax3-injected myogenic progenitors (black bars, primary recipients). Data are shown as mean ± SEM (n = 4 per group). **p < 0.01