Fig. 3
From: Exercise promotes satellite cell contribution to myofibers in a load-dependent manner
Nuclear reporter mice show high SC contribution during exercise. aPax7CreERT2 mice were crossed with RosaCAG-LSL-ntdTomato mice to genetically trace SC-derived nuclei. Upon tamoxifen treatment, SCs and SC-derived myonuclei will express nTomato (nTom). b Quantification of Hoechst+ and c nTom+ nuclei per 100 μm fiber length (11–16 single myofibers per mouse, n = 4 mice per group). d Represented pictures of single myofibers isolated from soleus. Statistics, one-way ANOVA test with Tukey correction for multiple comparisons (*p < 0.05; **p < 0.01; ***p < 0.001). Violin plot is shown (b, c). Each dot represents a single mouse. Scale bar, 25 μm