Fig. 5
From: Dnmt3b regulates DUX4 expression in a tissue-dependent manner in transgenic D4Z4 mice
DNA methylation at the D4Z4 repeat array, but not the chromatin compaction score, is affected by the Dnmt3bMommeD14 variant. a The average DNA methylation level of 10 CpG dinucleotides just distal to the D4Z4 repeat (the FasPas region) was not changed in tail DNA of D4Z4-2.5/Dnmt3bMommeD14 mice (postnatal day 15). In contrast, in spleen DNA a significant reduction in DNA methylation levels was detected in D4Z4-2.5/Dnmt3bMommeD14 mice compared to D4Z4-2.5 mice (P = 0.026). Each dot represents the average DNA methylation level of 10 CpG sites per mouse. Differences between groups were compared using a Student’s t test. *P < 0.05. n.s. = not significant. 2.5 = D4Z4-2.5; MD14 = Dnmt3bMommeD14. b The chromatin compaction score (H3K9me3 level corrected for H3K4me2 level) was not different in the spleen of D4Z4-2.5/Dnmt3bMommeD14 mice compared to D4Z4-2.5 mice (postnatal day 15) as measured by ChIP-qPCR analysis. Each dot represents one mouse. Statistical analysis was performed using a Student’s t test. n.s. = not significant. 2.5 = D4Z4-2.5; MD14 = Dnmt3bMommeD14. c Dnmt3b is not enriched at the D4Z4 repeat in the spleen of D4Z4-2.5 mice (postnatal day 15) as determined by ChIP-qPCR. The error bars represent the SEM for two biological replicates