Fig. 3
From: Pro-myogenic small molecules revealed by a chemical screen on primary muscle stem cells
CEP-701 improves muscle regeneration and satellite cell engraftment. a Experimental schematic detailing the systemic delivery of CEP-701 to adult (2–4 months old) C57BL/6 mice following CTX-induced TA muscle regeneration. b Cardiotoxin (CTX)-induced muscle damage and subsequent regeneration as evidenced by newly formed embryonic myosin-positive (eMHC+) fibers. Green, embryonic myosin; red, laminin; blue, Hoechst. Scale bar indicates 100 μm. c Quantification of the fold change in the mean cross-sectional area (CSA) of eMHC+ regenerating muscle fibers after CEP-701 treatment. The TA muscle was damaged with CTX, and animals were treated subcutaneously, twice a day with vehicle or 10 mg/kg CEP-701. After 4 days, the muscle was isolated, imaged, and analyzed. Error bars indicate SEM from 3 independent experiments. **p < 0.01 by an unpaired two-tailed t test assuming unequal variance. d Quantification of the fold change in satellite cells in the regenerating muscle following treatment with CEP-701. The TA muscle was damaged with CTX, and animals were treated subcutaneously, twice a day with vehicle or 10 mg/kg CEP-701. CXCR4+/ITGβ1+ satellite cells were isolated by FACS and quantified as a percentage of the total calcein AM+/propidium iodide- live cells. Error bars indicate SEM from 7 independent experiments. **p < 0.01 by an unpaired two-tailed t test assuming unequal variance. e Experimental schematic outlining ex vivo expansion of satellite cells in the presence of CEP-701 followed by transplantation into regenerating TA muscle of mdx mice. f Representative images of donor-derived GFP+ fibers (green) 3 weeks post-transplantation of CEP-701-treated satellite cells into CTX-injured TA muscle of mdx mice. Arrows designate donor-derived myofibers expressing GFP. Scale bar denotes 100 μm. g Quantification of engraftment efficiency based on the number of donor-derived myofibers expressing GFP per muscle section. Error bars indicate SEM from 5 independent experiments. **p < 0.01 by an unpaired two-tailed t test assuming unequal variance