Fig. 4

CSA, fiber-type specific CSA, and fiber-type distribution. Comparisons of manual analysis to the analyses from MyoSight. a MHC I fibers from 3 WT and 3 mdx mice analyzed either manually (WT: n = 574 fibers, mdx: n = 572 fibers) or with MyoSight (WT: n = 664 fibers, mdx: n = 657 fibers). b MHC IIa fibers from 3 WT and 3 mdx mice analyzed either manually (WT: n = 1155 fibers, mdx: n = 1165 fibers) or with MyoSight (WT: n = 1039 fibers, mdx: n = 968 fibers). c MHC IIx fibers from 3 WT and 3 mdx mice analyzed either manually (WT: n = 193 fibers, mdx: n = 178 fibers) or with MyoSight (WT: n = 358 fibers, mdx: n = 358 fibers). d Correlation of CSA of MHC I fibers from WT and mdx mice from MyoSight with the CSAs of these fibers assessed manually. e Correlation of CSA of MHC IIa fibers from WT and mdx mice from MyoSight with the CSAs of these fibers assessed manually. f Correlation of CSA of MHC IIx fibers from WT and mdx mice from MyoSight with the CSAs of these fibers assessed manually. g Distribution of type I fibers analyzed manually as a function of binned CSA for WT and mdx fibers (n = 3, mean ± SEM). h Distribution of MHC IIa fibers analyzed manually as a function of binned CSA for WT and mdx fibers (n = 3, mean ± SEM). i Distribution of MHC IIx fibers analyzed manually as a function of binned CSA for WT and mdx fibers (n = 3, mean ± SEM). j Distribution of MHC I fibers analyzed with MyoSight as a function of binned CSA for WT and mdx fibers (n = 3, mean ± SEM). k Distribution of MHC IIa fibers analyzed with MyoSight as a function of binned CSA for WT and mdx fibers (n = 3, mean ± SEM). l Distribution of type IIx fibers analyzed with MyoSight as a function of binned CSA for WT and mdx fibers (n = 3, mean ± SEM). m Fiber-type distribution determined from manual analysis (n = 3). n Fiber-type distribution determined by MyoSight analysis (n = 3). ND, not detected. Differences between WT and mdx represented by * p ≤ 0.05,** p ≤ 0.01,*** p ≤ 0.001, and **** p ≤ 0.0001