Fig. 1
CD82KO skeletal muscle shows no gross histological or functional abnormalities but has smaller myofibers. a, b H&E representative images of 8-week-old WT and CD82−/− tibialis anterior (TA) muscles show predominantly normal tissue in both strains. c Analyses of myofiber size via minimum Feret’s diameter measurements show significant difference in myofiber size between the two strains. Between 505 and 840 myofibers were measured per each animal, n = 6 animals/genotype. d Myofiber distribution plots of WT and CD82−/− at 8 weeks. e, f H&E representative images of 1-year-old WT and CD82−/− TA muscles, respectively. g Analyses of myofiber size via minimum Feret’s diameter measurements show significant difference between the two strains. h Myofiber distribution plots of WT and CD82−/− at 1 year. i Comparison of TA weight does not show significant differences between genotypes. j Peak force measurements normalized to CSA and k relative force measurements following serial tetanic stimulations do not show significant differences between genotypes. l Serum creatine kinase levels are normal and undistinguishable between WT and CD82−/− mice. Sera from mdx5cv mice were used as positive controls. Each dot represents an independent animal. ***p < 0.001; ****p < 0.0001. N = 6–10 animals/genotype in each experiment, with the exception of CK analyses (l), where a minimum of 20 mice were analyzed per genotype