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Fig. 3 | Skeletal Muscle

Fig. 3

From: Tetraspanin CD82 is necessary for muscle stem cell activation and supports dystrophic muscle function

Fig. 3

Satellite cell activation is transient in CD82−/− mice and Pax7 expression is retained. a, b Immunofluorescence staining of satellite cells for Pax7 (red) and MyoD (green) 72 h post-extraction from WT (a) and CD82−/− (b) animals, respectively (n = 6–10/genotype). Arrowhead in (b) points to Pax7+MyoD- cells that are more abundant in CD82−/− cultures. c Quantification of quiescent Pax7+MyoD satellite cells at 24, 48, and 72 h following isolation and d Pax7+MyoD+ activated satellite cells from WT and CD82−/− cultures at the same timepoints (**p ≤ 0.01; ****p ≤ 0.0001). eh Images and quantification of Pax7+Ki67 (g) and Pax7+Ki67+ (h) cells extracted from WT (e) and CD82−/− (f) cultures 5 days following isolation (*p < 0.05). Arrowheads in e and f point at Pax7+Ki67 cells, which are more abundant in CD82−/− cultures. i, j Flow cytometry cell cycle profiles of WT and CD82−/− myogenic cultures following expansion for ~ 30 days. 10,000 events were analyzed per each sample via FACS. k Quantification of percentage of cells in the S-phase and l in the G0/G1 phase from WT and CD82−/− cultures (*p ≤ 0.05; NS = not significant)

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