Fig. 7

IL-4 and STAT6 independently regulated myogenesis. Myoblasts were isolated from WT and STAT6-KO mice and maintained in DM with or without IL-4 treatment. a Representative immunostained myotubes positive for MyHC (green). Nuclei were stained with DAPI (blue). Scale bar = 50 μm. b Quantification of fusion index. n = 5. *p < .05. c Representative protein bands for p-STAT6, STAT6, p-p38 MAPK, p38 MAPK, and GAPDH. d p-STAT6 protein expression. n = 6. *p < .05 by unpaired t test. UD denotes undetected. e p-p38 MAPK protein expression. *p < .05. Data are presented as mean ± SD