Fig. 5

Confirmation of Six1 binding at the Slc16a10 enhancer. Chromatin from hindlimb muscles of four mice was harvested, was fixed, and ChIP was carried out using either anti-Six1 antibody or normal rabbit IgG. Results represent qPCR quantification of IP enrichment (expressed as percentage of input DNA) at the 48 kb binding site upstream of the Slc16a10/MCT10 locus (region corresponding to the pink box in Fig. 4A). Enrichment at the Myog proximal promoter is shown as positive control (region corresponding to the blue box in Fig. 4F) while lack of enrichment at the Hoxd10 proximal promoter is shown as specificity control. Results from each biological replicate are represented by different colors. Two-tailed paired T test were conducted to compare enrichment with IgG and with anti-Six1. One-tailed paired T tests also indicate that the degree of enrichment with anti-Six1 is significantly higher (p < 0.05) at the Myog and Slc16a10 loci compared to the Hoxd10 locus