Fig. 9

Direct binding of recombinant Sgcg N-terminus to the conserved archvillin/supervillin C-terminus. Supernatant depletion assays showed a significant loss of recombinant Sgcg-1-35-EGFP, but not of recombinant EGFP, after pull down with A, B GST-tagged bovine supervillin (bSV) residues 1398-1792 or C, D GST alone. Mutation of tyrosine-6 in Sgcg to alanine (Y6A) may affect this interaction. A, C Representative immunoblots of Input (I) and Unbound (U) fractions. B, D Densitometry quantification of replicate experiments. Statistical analyses employed one-way ANOVA, with a Tukey-Cramer multiple comparisons post-test. N = 3–5, as shown; *P < 0.05. Central lines, means; error bars, S.D.