Fig. 5

Confocal projection images of representative NMJ from the vastus muscle stained for postsynaptic AChR (BTX, A and D, red) and for nerve terminal vAChT (B and E, green). The merge is showing the colocalization of BTX and vAChT (C and F). Example is given of an innervated muscular NMJ where the BTX-stained area is largely covered by the vAChT staining (A–C). In contrast, D–F are showing a NMJ where the AChR-stained areas are only partially covered by vAChT staining, representing denervation. Scale bar in panel F is 10 μm. Mean postsynaptic (G) and presynaptic (H) NMJ areas and overlay areas of BTX and vAChT (I) in the vastus muscle are shown. Values are given as mean + SEM; n = 4 to 6 animals per group. ^##p < 0.01, significance between WT and iNOS^−/−