Fig. 3

The microgravity-induced transcriptome is muscle type-specific. Venn diagrams compare the total number of A differentially expressed and B differentially alternatively spliced genes in the gastrocnemius (blue) and quadriceps (red). The size of each circle is representative of the number of genes that were differentially expressed or differentially alternatively spliced in each muscle. Numerical values are provided for genes unique to each muscle as well as those that were held in common between the two muscles, represented by overlapping regions. Volcano plots display significance (− log₁₀ FDR) and fold change (log₂ FC) of differentially gene expression across ground control and flight mice in the C gastrocnemius D and quadriceps, labeled for non-significant (grey), upregulated (red), and downregulated (blue) genes. Volcano plots display significance (− log10 FDR) and change in percent spliced in (△PSI) of differential alternative splicing across ground control and flight mice in the E gastrocnemius F and quadriceps, labeled for non-significant (grey), included (red), and excluded (blue) exon skipping or mutually exclusive exon events